I første sæson forsøger 10 komikere (Silvia Abril, Edu Soto, Yolanda Ramos, Mario Vaquerizo, El Monaguillo, David Fernández, Arévalo, Rossy de Palma, Paco Collado og Carolina Noriega) at holde masken i seks timer samtidig med, at de skal få deres modstandere til at grine. Den exceptionelle vært, Santiago Segura, fører tilsyn med konkurrencen med hjælp fra Xavier Deltell.

Santiago Segura and his minion Deltell have kidnapped ten comedians to perform a crazy experiment. At the end, only one will remain.

Santiago surprises the comedians with an assignment that will bring about the first card for one of the favorites. A second ejection and three yellow cards will force the contestants to roll up their sleeves.

After two hours and five yellow cards, the comedians bring out their best weapons. Finally, a third contestant must leave the game.

It’s time to go all out to knock down their rivals. And they don’t disappoint… The competition is now down to five.

With only two hours left on the clock, the comedians have apparently lost their minds. A new ejection leaves only four contestants to face the last hour of the game.

A double ejection means there are only two contestants left in the game. In a final push, they challenge each other, trying to make their rival crack. After an intense duel, one of them will be declared the first winner on LOL “If you laugh, you lose”.